› 6zht

X-ray diffraction
2.3Å resolution

Uba1-Ubc13 disulfide mediated complex

Released:
DOI: 10.2210/pdb6zht/pdb
PDB entry 6zht coloured by chain, front view.
PDB entry 6zht coloured by chain, side view.
PDB entry 6zht coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Schaefer A, Misra M, Schindelin H

Function and Biology Details

Reactions catalysed: 
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149659 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-activating enzyme E1 1 Chain: C
Molecule details ›
Chain: C
Length: 1001 amino acids
Theoretical weight: 111.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P22515 (Residues: 25-1024; Coverage: 98%)
Gene names: UBA1, YKL210W
Sequence domains:
Ubiquitin-conjugating enzyme E2 13 Chain: B
Molecule details ›
Chain: B
Length: 153 amino acids
Theoretical weight: 17.42 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P52490 (Residues: 1-152; Coverage: 99%)
Gene names: UBC13, YD6652.04, YDR092W
Sequence domains: Ubiquitin-conjugating enzyme

Ligands and Environments

2 bound ligands:
Ligand GOL 12 x GOL
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 181.39Å b: 58.27Å c: 137.46Å
α: 90° β: 109.72° γ: 90°
R-values:
R R work R free
0.209 0.206 0.263
Expression system: Escherichia coli

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