› 6zjn

Electron Microscopy
6.1Å resolution

Respiratory complex I from Thermus thermophilus, NADH dataset, minor state

Released:
DOI: 10.2210/pdb6zjn/pdb
PDB entry 6zjn coloured by chain, front view.
PDB entry 6zjn coloured by chain, side view.
PDB entry 6zjn coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Key role of quinone in the mechanism of respiratory complex I.
Gutiérrez-Fernández J, Kaszuba K, Minhas GS, Baradaran R, Tambalo M, Gallagher DT, Sazanov LA
OpenAccess logo Nat Commun 11 4135 (2020)
PMID: 32811817
Related structures: EMD-11237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176329 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
NADH-quinone oxidoreductase subunit 1 Chain: 1
Molecule details ›
Chain: 1
Length: 438 amino acids
Theoretical weight: 48.69 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56222 (Residues: 1-438; Coverage: 100%)
Gene names: TTHA0089, nqo1
Sequence domains:
NADH-quinone oxidoreductase subunit 2 Chain: 2
Molecule details ›
Chain: 2
Length: 181 amino acids
Theoretical weight: 20.31 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q56221 (Residues: 1-181; Coverage: 100%)
Gene names: TTHA0088, nqo2
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase subunit 3 Chain: 3
NADH-quinone oxidoreductase subunit 4 Chain: 4
NADH-quinone oxidoreductase subunit 5 Chain: 5
NADH-quinone oxidoreductase subunit 6 Chain: 6
NADH-quinone oxidoreductase subunit 9 Chain: 9
NADH-quinone oxidoreductase subunit 15 Chain: 7
NADH-quinone oxidoreductase subunit 7 Chain: A
NADH-quinone oxidoreductase subunit 10 Chain: J
NADH-quinone oxidoreductase subunit 11 Chain: K
NADH-quinone oxidoreductase subunit 12 Chain: L
NADH-quinone oxidoreductase subunit 13 Chain: M
NADH-quinone oxidoreductase subunit 14 Chain: N
NADH-quinone oxidoreductase subunit 8 Chain: H

Ligands and Environments

2 bound ligands:
Ligand SF4 7 x SF4
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 6.1Å
Relevant EMDB volumes: EMD-11237

Quick links

Citations

8 review citations

The assembly, regulation and function of the mitochondrial respiratory chain.
Vercellino et al. (2022)
7 more

1 mention without citation

DiffModeler: Large Macromolecular Structure Modeling in Low-Resolution Cryo-EM Maps Using Diffusion Model.
Wang et al. (2024)