› 6zl6

X-ray diffraction
1.7Å resolution

Crystal Structure of UDP-Glucuronic acid 4-epimerase from Bacillus cereus in complex with UDP and NAD

Released:
DOI: 10.2210/pdb6zl6/pdb
PDB entry 6zl6 coloured by chain, front view.
PDB entry 6zl6 coloured by chain, side view.
PDB entry 6zl6 coloured by chain, top view.
Source organism: Bacillus cereus HuA2-4
Primary publication:
Crystallographic snapshots of UDP-glucuronic acid 4-epimerase ligand binding, rotation, and reduction.
Iacovino LG, Savino S, Borg AJE, Binda C, Nidetzky B, Mattevi A
J Biol Chem 295 12461-12473 (2020)
PMID: 32661196

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epimerase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 36.53 KDa
Source organism: Bacillus cereus HuA2-4
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand UDP 2 x UDP
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 42.482Å b: 58.56Å c: 64.904Å
α: 96.77° β: 98.41° γ: 110.3°
R-values:
R R work R free
0.172 0.171 0.202
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Enzymatic C4-Epimerization of UDP-Glucuronic Acid: Precisely Steered Rotation of a Transient 4-Keto Intermediate for an Inverted Reaction without Decarboxylation.
Borg et al. (2023)
1 more

1 mention without citation

Crystallographic snapshots of UDP-glucuronic acid 4-epimerase ligand binding, rotation, and reduction.
Iacovino et al. (2020)