› 6zmn

X-ray diffraction
2.33Å resolution

Crystal structure of the Smad3-Smad5 MH1 domain chimera bound to the GGCGC site

Released:
DOI: 10.2210/pdb6zmn/pdb
PDB entry 6zmn coloured by chain, front view.
PDB entry 6zmn coloured by chain, side view.
PDB entry 6zmn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes.
Ruiz L, Kaczmarska Z, Gomes T, Aragon E, Torner C, Freier R, Baginski B, Martin-Malpartida P, de Martin Garrido N, Marquez JA, Cordeiro TN, Pluta R, Macias MJ
Comput Struct Biotechnol J 19 632-646 (2021)
PMID: 33510867

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121021 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 14.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P84022 (Residues: 10-136; Coverage: 29%)
Gene names: MADH3, SMAD3
Sequence domains: MH1 domain
DNA (5'-D(P*TP*GP*CP*AP*GP*GP*CP*GP*CP*GP*CP*CP*TP*GP*CP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand ACT 1 x ACT
Ligand EDO 1 x EDO
Ligand ZN 2 x ZN
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 54.502Å b: 73.416Å c: 111.154Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.252
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Canonical TGFβ Signaling and Its Contribution to Endometrial Cancer Development and Progression-Underestimated Target of Anticancer Strategies.
Zakrzewski PK. (2021)
2 other articles

1 mention without citation

Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes.
Ruiz et al. (2021)