› 6znn

Electron Microscopy
4.5Å resolution

The pointed end complex of dynactin bound to Hook3

Released:
DOI: 10.2210/pdb6znn/pdb
PDB entry 6znn coloured by chain, front view.
PDB entry 6znn coloured by chain, side view.
PDB entry 6znn coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end.
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J 40 e106164 (2021)
PMID: 33734450
Related structures: EMD-11318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-104022 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Alpha-centractin Chains: G, I
Molecule details ›
Chains: G, I
Length: 376 amino acids
Theoretical weight: 42.67 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F2Z5G5 (Residues: 1-310; Coverage: 89%)
Gene name: ACTR1A
Sequence domains: Actin
Actin, cytoplasmic 1 Chain: H
Molecule details ›
Chain: H
Length: 375 amino acids
Theoretical weight: 41.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q6QAQ1 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Actin-related protein 10 Chain: J
Molecule details ›
Chain: J
Length: 417 amino acids
Theoretical weight: 46.25 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LHK5 (Residues: 1-417; Coverage: 100%)
Gene names: ACTR10, ARP11
Sequence domains: Actin
Dynactin subunit 2 Chain: M
Molecule details ›
Chain: M
Length: 405 amino acids
Theoretical weight: 44.7 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A5G2QD80 (Residues: 1-405; Coverage: 100%)
Gene name: DCTN2
Sequence domains: Dynamitin
putative Hook3 coiled coil Chains: S, T
Molecule details ›
Chains: S, T
Length: 68 amino acids
Theoretical weight: 5.81 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Dynactin subunit 6 Chain: U
Molecule details ›
Chain: U
Length: 190 amino acids
Theoretical weight: 20.7 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: D0G6S1 (Residues: 1-190; Coverage: 100%)
Gene name: DCTN6
Dynactin subunit 5 Chain: V
Molecule details ›
Chain: V
Length: 182 amino acids
Theoretical weight: 20.15 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZK88 (Residues: 1-182; Coverage: 100%)
Gene name: DCTN5
Sequence domains: Dynactin subunit 5
Hook3 Chain: X
Molecule details ›
Chain: X
Length: 93 amino acids
Theoretical weight: 7.93 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Dynactin subunit 4 Chain: Y
Molecule details ›
Chain: Y
Length: 467 amino acids
Theoretical weight: 52.92 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1TB62 (Residues: 1-467; Coverage: 100%)
Gene name: DCTN4
Sequence domains: Dynactin p62 family
Hook3 Chain: x
Molecule details ›
Chain: x
Length: 92 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

3 bound ligands:
Ligand ADP 3 x ADP
Ligand ATP 1 x ATP
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-11318
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Multiple roles for the cytoskeleton in ALS.
Liu et al. (2022)
1 more

1 mention without citation

Status and Opportunities of Zinc Ion Hybrid Capacitors: Focus on Carbon Materials, Current Collectors, and Separators.
Wang et al. (2023)