› 6zsu

X-ray diffraction
2Å resolution

Structure of crocagin biosynthetic protein CgnE

Released:
DOI: 10.2210/pdb6zsu/pdb
PDB entry 6zsu coloured by chain, front view.
PDB entry 6zsu coloured by chain, side view.
PDB entry 6zsu coloured by chain, top view.
Source organism: Chondromyces crocatus
Primary publication:
Unusual peptide-binding proteins guide pyrroloindoline alkaloid formation in crocagin biosynthesis.
Adam S, Zheng D, Klein A, Volz C, Mullen W, Shirran SL, Smith BO, Kalinina OV, Müller R, Koehnke J
Nat Chem (2023)
PMID: 36894702

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CgnE Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 34.98 KDa
Source organism: Chondromyces crocatus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K1ECI7 (Residues: 1-309; Coverage: 100%)
Gene name: CMC5_025530

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 70.4Å b: 75.34Å c: 112.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.202
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structure and Function of a Class III Metal-Independent Lanthipeptide Synthetase.
Hernandez Garcia et al. (2023)