› 6zsv

X-ray diffraction
2.3Å resolution

Structure of crocagin biosynthetic protein CgnB

Released:
DOI: 10.2210/pdb6zsv/pdb
PDB entry 6zsv coloured by chain, front view.
PDB entry 6zsv coloured by chain, side view.
PDB entry 6zsv coloured by chain, top view.
Source organism: Chondromyces crocatus
Primary publication:
Unusual peptide-binding proteins guide pyrroloindoline alkaloid formation in crocagin biosynthesis.
Adam S, Zheng D, Klein A, Volz C, Mullen W, Shirran SL, Smith BO, Kalinina OV, Müller R, Koehnke J
Nat Chem (2023)
PMID: 36894702

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.47 KDa
Source organism: Chondromyces crocatus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K1EBZ5 (Residues: 1-309; Coverage: 100%)
Gene name: CMC5_025570

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 52.448Å b: 67.5Å c: 86.152Å
α: 90° β: 95.99° γ: 90°
R-values:
R R work R free
0.284 0.283 0.305
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structure and Function of a Class III Metal-Independent Lanthipeptide Synthetase.
Hernandez Garcia et al. (2023)