› 6zti

X-ray diffraction
1.81Å resolution

Phospholipase PlaB from Legionella pneumophila in complex with thio-NAD

Released:
DOI: 10.2210/pdb6zti/pdb
PDB entry 6zti coloured by chain, front view.
PDB entry 6zti coloured by chain, side view.
PDB entry 6zti coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB.
Diwo M, Michel W, Aurass P, Kuhle-Keindorf K, Pippel J, Krausze J, Wamp S, Lang C, Blankenfeldt W, Flieger A
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34074754

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PlaB phospholipase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 489 amino acids
Theoretical weight: 55.39 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A378K488 (Residues: 1-474; Coverage: 100%)
Gene names: NCTC12000_01733, plaB

Ligands and Environments

2 bound ligands:
Ligand SND 8 x SND
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 75.831Å b: 90.725Å c: 92.79Å
α: 78.44° β: 90.26° γ: 75.41°
R-values:
R R work R free
0.188 0.186 0.234
Expression system: Escherichia coli

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Citations

1 mention without citation

NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB.
Diwo et al. (2021)