› 6ztu

X-ray diffraction
1.69Å resolution

Crystal structure of a cyclodipeptide synthase from Bacillus thermoamylovorans

Released:
DOI: 10.2210/pdb6ztu/pdb
PDB entry 6ztu coloured by chain, front view.
PDB entry 6ztu coloured by chain, side view.
PDB entry 6ztu coloured by chain, top view.
Source organism: Caldibacillus thermoamylovorans
Primary publication:
Bypassing the requirement for aminoacyl-tRNA by a cyclodipeptide synthase enzyme.
Harding CJ, Sutherland E, Hanna JG, Houston DR, Czekster CM
RSC Chem Biol 2 230-240 (2021)
PMID: 33937777

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100705 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclodipeptide synthase Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 27.26 KDa
Source organism: Caldibacillus thermoamylovorans
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A090KS30 (Residues: 1-231; Coverage: 100%)
Gene names: BT1A1_1686, yvmC
Sequence domains: Cyclodipeptide synthase

Ligands and Environments

1 bound ligand:
Ligand 0RE 1 x 0RE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 79.969Å b: 79.969Å c: 92.073Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.212
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Directed evolution of a cyclodipeptide synthase with new activities via label-free mass spectrometric screening.
Zhang et al. (2022)
2 more

2 mentions without citation

Bypassing the requirement for aminoacyl-tRNA by a cyclodipeptide synthase enzyme.
Harding et al. (2021)
1 more