› 6zvw

X-ray diffraction
1.6Å resolution

X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165H LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE

Released:
DOI: 10.2210/pdb6zvw/pdb
PDB entry 6zvw coloured by chain, front view.
PDB entry 6zvw coloured by chain, side view.
PDB entry 6zvw coloured by chain, top view.
Source organism: Rhodococcus sp.
Entry authors: Tarnawski M, Frei M, Hiblot J, Johnsson K

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 33.24 KDa
Source organism: Rhodococcus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3G3 (Residues: 4-293; Coverage: 99%)
Gene name: dhaA
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

4 bound ligands:
Ligand OEH 2 x OEH
Ligand CL 2 x CL
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 44.18Å b: 47.56Å c: 78.25Å
α: 97.373° β: 89.971° γ: 114.256°
R-values:
R R work R free
0.224 0.222 0.266
Expression system: Escherichia coli

Quick links