› 6zvx

X-ray diffraction
1.4Å resolution

X-RAY STRUCTURE OF THE HALOALKANE DEHALOGENASE HALOTAG7-Q165H-P174L LABELED WITH A CHLOROALKANE-TETRAMETHYLRHODAMINE FLUOROPHORE SUBSTRATE

Released:
DOI: 10.2210/pdb6zvx/pdb
PDB entry 6zvx coloured by chain, front view.
PDB entry 6zvx coloured by chain, side view.
PDB entry 6zvx coloured by chain, top view.
Source organism: Rhodococcus sp.
Entry authors: Tarnawski M, Frei M, Hiblot J, Johnsson K

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.25 KDa
Source organism: Rhodococcus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3G3 (Residues: 4-293; Coverage: 99%)
Gene name: dhaA
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

3 bound ligands:
Ligand OEH 1 x OEH
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 44.15Å b: 78.21Å c: 44.53Å
α: 90° β: 118.462° γ: 90°
R-values:
R R work R free
0.141 0.14 0.159
Expression system: Escherichia coli

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