› 6zvz

X-ray diffraction
2.3Å resolution

Connectase MJ0548 from Methanocaldococcus jannaschii

Released:
DOI: 10.2210/pdb6zvz/pdb
PDB entry 6zvz coloured by chain, front view.
PDB entry 6zvz coloured by chain, side view.
PDB entry 6zvz coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Archaeal Connectase is a specific and efficient protein ligase related to proteasome β subunits.
Fuchs ACD, Ammelburg M, Martin J, Schmitz RA, Hartmann MD, Lupas AN
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33688044

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Connectase MJ0548 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 295 amino acids
Theoretical weight: 34.08 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57968 (Residues: 1-293; Coverage: 100%)
Gene name: MJ0548
Sequence domains: Uncharacterized protein conserved in archaea (DUF2121)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 76.162Å b: 190.221Å c: 111.863Å
α: 90° β: 107.43° γ: 90°
R-values:
R R work R free
0.244 0.242 0.277
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Design and Evolution of Enhanced Peptide-Peptide Ligation for Modular Transglutaminase Assembly.
Keeble et al. (2023)
2 more

1 mention without citation

An astonishing wealth of new proteasome homologs.
Fuchs et al. (2021)