› 6zxt

X-ray diffraction
1.4Å resolution

High resolution crystal structure of chloroplastic ribose-5-phosphate isomerase from Chlamydomonas reinhardtii

Released:
DOI: 10.2210/pdb6zxt/pdb
Model geometry
Fit model/data
PDB entry 6zxt coloured by chain, front view.
PDB entry 6zxt coloured by chain, side view.
PDB entry 6zxt coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
High-Resolution Crystal Structure of Chloroplastic Ribose-5-Phosphate Isomerase from Chlamydomonas reinhardtii-An Enzyme Involved in the Photosynthetic Calvin-Benson Cycle.
Le Moigne T, Crozet P, Lemaire SD, Henri J
OpenAccess logo Int J Mol Sci 21 (2020)
PMID: 33096784

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ribose-5-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.59 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8IRQ1 (Residues: 23-269; Coverage: 92%)
Gene name: CHLRE_03g187450v5
Sequence domains: Ribose 5-phosphate isomerase A (phosphoriboisomerase A)

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 45.152Å b: 63.952Å c: 80.909Å
α: 90° β: 96.13° γ: 90°
R-values:
R R work R free
0.158 0.158 0.176
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Physiological Biochemistry-Combined Transcriptomic Analysis Reveals Mechanism of Bacillus cereus G2 Improved Salt-Stress Tolerance of Glycyrrhiza uralensis Fisch. Seedlings by Balancing Carbohydrate Metabolism.
Xiao et al. (2021)

2 mentions without citation

Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate.
Meloni et al. (2023)
1 more