› 6zyf

X-ray diffraction
2.19Å resolution

Notum_Ghrelin complex

Released:
DOI: 10.2210/pdb6zyf/pdb
PDB entry 6zyf coloured by chain, front view.
PDB entry 6zyf coloured by chain, side view.
PDB entry 6zyf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Notum deacylates octanoylated ghrelin.
Zhao Y, Schuhmacher LN, Roberts M, Kakugawa S, Bineva-Todd G, Howell S, O'Reilly N, Perret C, Snijders AP, Vincent JP, Jones EY
Mol Metab 49 101201 (2021)
PMID: 33647468

Function and Biology Details

Reaction catalysed: 
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine + H(2)O = [Wnt]-L-serine + (9Z)-hexadec-9-enoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Palmitoleoyl-protein carboxylesterase NOTUM Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 43.55 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6P988 (Residues: 81-451; Coverage: 78%)
Gene names: NOTUM, OK/SW-CL.30
Sequence domains: Pectinacetylesterase

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand K4Q 2 x K4Q
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21212
Unit cell:
a: 75.418Å b: 184.072Å c: 61.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.252
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Ghrelin octanoylation by ghrelin O-acyltransferase: protein acylation impacting metabolic and neuroendocrine signalling.
Davis et al. (2021)
2 more

2 mentions without citation

Small-molecule inhibitors of carboxylesterase Notum.
Zhao et al. (2021)
1 more