› 6zyl

X-ray diffraction
2.09Å resolution

non-heme monooxygenase; ThoJ apo

Released:
DOI: 10.2210/pdb6zyl/pdb
PDB entry 6zyl coloured by chain, front view.
PDB entry 6zyl coloured by chain, side view.
PDB entry 6zyl coloured by chain, top view.
Source organism: Streptomyces malaysiense
Primary publication:
Non-Heme Monooxygenase ThoJ Catalyzes Thioholgamide β-Hydroxylation.
Sikandar A, Lopatniuk M, Luzhetskyy A, Koehnke J
ACS Chem Biol 15 2815-2819 (2020)
PMID: 32965102

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytanoyl-CoA dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 33.58 KDa
Source organism: Streptomyces malaysiense
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A1J4PXK4 (Residues: 1-290; Coverage: 100%)
Gene name: VT52_024865
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)

Ligands and Environments

1 bound ligand:
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P41212
Unit cell:
a: 80.517Å b: 80.517Å c: 130.144Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.207
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 citations in other articles

Understanding thioamitide biosynthesis using pathway engineering and untargeted metabolomics.
Eyles et al. (2021)