› 6zzx

Electron Microscopy
2.7Å resolution

Structure of low-light grown Chlorella ohadii Photosystem I

Released:
DOI: 10.2210/pdb6zzx/pdb
PDB entry 6zzx coloured by chain, front view.
PDB entry 6zzx coloured by chain, side view.
PDB entry 6zzx coloured by chain, top view.
Source organism: Chlorella ohadii
Primary publication:
Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii.
Caspy I, Neumann E, Fadeeva M, Liveanu V, Savitsky A, Frank A, Kalisman YL, Shkolnisky Y, Murik O, Treves H, Hartmann V, Nowaczyk MM, Schuhmann W, Rögner M, Willner I, Kaplan A, Schuster G, Nelson N, Lubitz W, Nechushtai R
Nat Plants 7 1314-1322 (2021)
PMID: 34462576
Related structures: EMD-11588

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-104393 (preferred)
Entry contents:
23 distinct polypeptide molecules
Macromolecules (23 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chain: A
Molecule details ›
Chain: A
Length: 741 amino acids
Theoretical weight: 82.13 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SY74 (Residues: 11-751; Coverage: 99%)
Gene names: csorokcp_00027, psaA
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chain: B
Molecule details ›
Chain: B
Length: 731 amino acids
Theoretical weight: 81.54 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SUA3 (Residues: 5-733; Coverage: 99%)
Gene names: csorokcp_00028, psaB
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I iron-sulfur center Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 8.72 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SKM2 (Residues: 2-81; Coverage: 99%)
Gene names: csorokcp_00038, psaC
Sequence domains: 4Fe-4S dicluster domain
Photosystem I reaction center subunit chloroplastic Chain: D
Molecule details ›
Chain: D
Length: 143 amino acids
Theoretical weight: 15.96 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TKF8 (Residues: 188-330; Coverage: 43%)
Gene name: C2E21_6785
Sequence domains: PsaD
Photosystem I reaction center subunit IV Chain: E
Molecule details ›
Chain: E
Length: 64 amino acids
Theoretical weight: 7.19 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6U4S6 (Residues: 35-98; Coverage: 64%)
Gene name: C2E21_0167
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III Chain: F
Molecule details ›
Chain: F
Length: 165 amino acids
Theoretical weight: 18.07 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TPV8 (Residues: 318-482; Coverage: 34%)
Gene name: C2E21_5099
Sequence domains: Photosystem I reaction centre subunit III
PSI-G Chain: G
Molecule details ›
Chain: G
Length: 99 amino acids
Theoretical weight: 10.33 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TZI8 (Residues: 1228-1326; Coverage: 8%)
Gene name: C2E21_1494
Sequence domains: Photosystem I psaG / psaK
Photosystem I reaction center subunit VI-chloroplastic-like Chain: H
Molecule details ›
Chain: H
Length: 94 amino acids
Theoretical weight: 10.32 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TPU7 (Residues: 34-128; Coverage: 73%)
Gene name: C2E21_5226
Sequence domains: Photosystem I reaction centre subunit VI
Photosystem I reaction center subunit IX Chain: J
Molecule details ›
Chain: J
Length: 41 amino acids
Theoretical weight: 4.48 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SUD1 (Residues: 1-41; Coverage: 100%)
Gene names: csorokcp_00066, psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
PSI-K Chain: K
Molecule details ›
Chain: K
Length: 86 amino acids
Theoretical weight: 8.73 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6U0J1 (Residues: 32-117; Coverage: 74%)
Gene name: C2E21_1903
Sequence domains: Photosystem I psaG / psaK
PSI subunit V Chain: L
Molecule details ›
Chain: L
Length: 157 amino acids
Theoretical weight: 16.5 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TC44 (Residues: 295-452; Coverage: 35%)
Gene name: C2E21_9213
Sequence domains: Photosystem I reaction centre subunit XI
Photosystem I reaction center subunit XII Chain: M
Molecule details ›
Chain: M
Length: 31 amino acids
Theoretical weight: 3.38 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SU98 (Residues: 1-31; Coverage: 100%)
Gene names: csorokcp_00017, psaM
Sequence domains: Photosystem I protein M (PsaM)
Photosystem I reaction center subunit VIII Chain: I
Molecule details ›
Chain: I
Length: 35 amino acids
Theoretical weight: 3.86 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SIP9 (Residues: 1-35; Coverage: 97%)
Gene names: csorokcp_00005, psaI
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I subunit O Chain: O
Molecule details ›
Chain: O
Length: 87 amino acids
Theoretical weight: 9.55 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6THB2 (Residues: 47-133; Coverage: 64%)
Gene name: C2E21_7569
Chlorophyll a-b binding protein, chloroplastic Chains: 1, a
Molecule details ›
Chains: 1, a
Length: 192 amino acids
Theoretical weight: 19.93 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TT36 (Residues: 32-223; Coverage: 86%)
Gene name: C2E21_4122
Sequence domains: Chlorophyll A-B binding protein
Glutathione reductase Chain: 3
Molecule details ›
Chain: 3
Length: 241 amino acids
Theoretical weight: 26.16 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TMT4 (Residues: 247-487; Coverage: 12%)
Gene name: C2E21_6000
Sequence domains: Chlorophyll A-B binding protein
Lhca4 Chain: 4
Molecule details ›
Chain: 4
Length: 207 amino acids
Theoretical weight: 23.01 KDa
Source organism: Chlorella ohadii
Chlorophyll a-b binding protein, chloroplastic Chain: 5
Molecule details ›
Chain: 5
Length: 229 amino acids
Theoretical weight: 25.37 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6U4K1 (Residues: 30-258; Coverage: 88%)
Gene name: C2E21_0580
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 6
Molecule details ›
Chain: 6
Length: 231 amino acids
Theoretical weight: 25.32 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TPR7 (Residues: 35-265; Coverage: 87%)
Gene name: C2E21_5184
Sequence domains: Chlorophyll A-B binding protein
Lhca7 Chain: 7
Molecule details ›
Chain: 7
Length: 221 amino acids
Theoretical weight: 24.02 KDa
Source organism: Chlorella ohadii
Chlorophyll a-b binding protein, chloroplastic Chain: 8
Molecule details ›
Chain: 8
Length: 219 amino acids
Theoretical weight: 23.61 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TZ50 (Residues: 29-247; Coverage: 88%)
Gene name: C2E21_2201
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 2
Molecule details ›
Chain: 2
Length: 215 amino acids
Theoretical weight: 23.62 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TMX4 (Residues: 30-244; Coverage: 88%)
Gene name: C2E21_5760
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 9
Molecule details ›
Chain: 9
Length: 183 amino acids
Theoretical weight: 19.91 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TMI2 (Residues: 545-727; Coverage: 13%)
Gene name: C2E21_5761
Sequence domains: Chlorophyll A-B binding protein

Ligands and Environments

30 bound ligands:
Ligand CL0 1 x CL0
Ligand CLA 208 x CLA
Ligand CHL 47 x CHL
Ligand PQN 2 x PQN
Ligand SF4 3 x SF4
Ligand BCR 32 x BCR
Ligand LHG 24 x LHG
Ligand LMG 1 x LMG
Ligand SQD 5 x SQD
Ligand PTY 11 x PTY
Ligand 3PH 1 x 3PH
Ligand LMT 4 x LMT
Ligand DGD 4 x DGD
Ligand PCW 4 x PCW
Ligand LPX 1 x LPX
Ligand NEX 1 x NEX
Ligand RRX 2 x RRX
Ligand SPH 4 x SPH
Ligand ECH 1 x ECH
Ligand LUT 21 x LUT
Ligand AXT 2 x AXT
Ligand OLA 4 x OLA
Ligand QTB 1 x QTB
Ligand PLM 7 x PLM
Ligand DGA 4 x DGA
Ligand 13X 1 x 13X
Ligand XAT 4 x XAT
Ligand 4RF 1 x 4RF
Ligand P5S 1 x P5S
Ligand LAP 1 x LAP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-11588

Quick links

Citations

2 review citations

Harnessing photosynthesis to produce electricity using cyanobacteria, green algae, seaweeds and plants.
Shlosberg et al. (2022)
1 more