› 7a0d

X-ray diffraction
1.6Å resolution

The Crystal Structure of Bovine Thrombin in complex with Hirudin (C16U/C28U) at 1.6 Angstroms Resolution

Released:
DOI: 10.2210/pdb7a0d/pdb
Model geometry
Fit model/data
PDB entry 7a0d coloured by chain, front view.
PDB entry 7a0d coloured by chain, side view.
PDB entry 7a0d coloured by chain, top view.
Source organisms:
Primary publication:
Diselenide crosslinks for enhanced and simplified oxidative protein folding
Mousa R, Hidmi T, Pomyalov S, Lansky S, Khouri L, Shalev DE, Shoham G, Metanis N
Commun Chem 4 30- (2021)

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133168 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Thrombin heavy chain Chain: HHH
Molecule details ›
Chain: HHH
Length: 259 amino acids
Theoretical weight: 29.77 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00735 (Residues: 367-625; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Hirudin variant-1 Chain: III
Molecule details ›
Chain: III
Length: 65 amino acids
Theoretical weight: 7.07 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P01050 (Residues: 1-65; Coverage: 100%)
Sequence domains: Hirudin
Thrombin light chain Chain: LLL

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2221
Unit cell:
a: 58.672Å b: 102.374Å c: 142.735Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.215
Expression system: Not provided

Quick links