› 7a2x

X-ray diffraction
0.92Å resolution

Crystal structure of the Fyn SH3 domain L112V-S114N-S115T-E121L-R123H mutant in complex with VSL12 at pH 5.0

Released:
DOI: 10.2210/pdb7a2x/pdb
PDB entry 7a2x coloured by chain, front view.
PDB entry 7a2x coloured by chain, side view.
PDB entry 7a2x coloured by chain, top view.
Source organisms:
Entry authors: Camara-Artigas A, Plaza-Garrido M, Salinas-Garcia MC

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138969 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Fyn Chain: A
Molecule details ›
Chain: A
Length: 60 amino acids
Theoretical weight: 6.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06241 (Residues: 83-142; Coverage: 11%)
Gene name: FYN
Sequence domains: SH3 domain
VSL12 high affinity synthetic peptide acetylated in the amino-terminus Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.34 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 32.897Å b: 33.1Å c: 62.624Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.13 0.148
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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