› 7a3p

X-ray diffraction
3.19Å resolution

Crystal structure of dengue 3 virus envelope glycoprotein in complex with the scFv fragment of the broadly neutralizing human antibody EDE1 C10

Released:
DOI: 10.2210/pdb7a3p/pdb
PDB entry 7a3p coloured by chain, front view.
PDB entry 7a3p coloured by chain, side view.
PDB entry 7a3p coloured by chain, top view.
Source organisms:
Primary publication:
The epitope arrangement on flavivirus particles contributes to Mab C10's extraordinary neutralization breadth across Zika and dengue viruses.
Sharma A, Zhang X, Dejnirattisai W, Dai X, Gong D, Wongwiwat W, Duquerroy S, Rouvinski A, Vaney MC, Guardado-Calvo P, Haouz A, England P, Sun R, Zhou ZH, Mongkolsapaya J, Screaton GR, Rey FA
Cell 184 6052-6066.e18 (2021)
PMID: 34852239

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208072 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope protein E Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 46.82 KDa
Source organism: dengue virus type 3
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O39791 (Residues: 281-673; Coverage: 12%)
Sequence domains:
Single Chain Variable Fragment Chains: H, I
Molecule details ›
Chains: H, I
Length: 144 amino acids
Theoretical weight: 15.68 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
Single Chain Variable Fragment Chains: L, M
Molecule details ›
Chains: L, M
Length: 154 amino acids
Theoretical weight: 15.63 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 53.744Å b: 101.412Å c: 249.236Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.24 0.284
Expression system: Drosophila melanogaster

Quick links

Citations

5 review citations

Current Advances in Zika Vaccine Development.
Wang et al. (2022)
4 more

1 mention without citation

The epitope arrangement on flavivirus particles contributes to Mab C10's extraordinary neutralization breadth across Zika and dengue viruses.
Sharma et al. (2021)