› 7a48

X-ray diffraction
1.55Å resolution

Crystal structure of the APH coiled-coil in complex with Nb49

Released:
DOI: 10.2210/pdb7a48/pdb
PDB entry 7a48 coloured by chain, front view.
PDB entry 7a48 coloured by chain, side view.
PDB entry 7a48 coloured by chain, top view.
Source organisms:
Primary publication:
A nanobody toolbox targeting dimeric coiled-coil modules for functionalization of designed protein origami structures.
Majerle A, Hadži S, Aupič J, Satler T, Lapenta F, Strmšek Ž, Lah J, Loris R, Jerala R
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33893235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-211784 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nanobody 49 Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.65 KDa
Source organism: Lama glama
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
APH colied-coil Chain: B
Molecule details ›
Chain: B
Length: 42 amino acids
Theoretical weight: 4.84 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 94.58Å b: 26.07Å c: 54.25Å
α: 90° β: 108.92° γ: 90°
R-values:
R R work R free
0.18 0.179 0.212
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

3 review citations

Understanding a protein fold: The physics, chemistry, and biology of α-helical coiled coils.
Woolfson DN. (2023)
2 more