› 7a4d

X-ray diffraction
2.69Å resolution

Crystal structure of the APH coiled-coil in complex with nanobodies Nb28 and Nb30

Released:
DOI: 10.2210/pdb7a4d/pdb
Model geometry
Fit model/data
PDB entry 7a4d coloured by chain, front view.
PDB entry 7a4d coloured by chain, side view.
PDB entry 7a4d coloured by chain, top view.
Source organisms:
Primary publication:
A nanobody toolbox targeting dimeric coiled-coil modules for functionalization of designed protein origami structures.
Majerle A, Hadži S, Aupič J, Satler T, Lapenta F, Strmšek Ž, Lah J, Loris R, Jerala R
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 33893235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-211785 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nanobody Nb28 Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 133 amino acids
Theoretical weight: 14.43 KDa
Source organism: Lama glama
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Nanobody Nb30 Chains: C, D, I, J
Molecule details ›
Chains: C, D, I, J
Length: 130 amino acids
Theoretical weight: 14.3 KDa
Source organism: Lama glama
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
APH coiled-coil Chains: E, F, K, L

Ligands and Environments

3 bound ligands:
Ligand EDO 3 x EDO
Ligand GOL 1 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 61.26Å b: 127.45Å c: 192.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.223
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

3 review citations

Understanding a protein fold: The physics, chemistry, and biology of α-helical coiled coils.
Woolfson DN. (2023)
2 more