› 7a4u

X-ray diffraction
1.77Å resolution

Crystal structure of lid-truncated apo BiP in an oligomeric state

Released:
DOI: 10.2210/pdb7a4u/pdb
PDB entry 7a4u coloured by chain, front view.
PDB entry 7a4u coloured by chain, side view.
PDB entry 7a4u coloured by chain, top view.
Source organism: Cricetulus griseus
Primary publication:
Calcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes.
Preissler S, Rato C, Yan Y, Perera LA, Czako A, Ron D
Elife 9 (2020)
PMID: 33295873

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoplasmic reticulum chaperone BiP Chain: A
Molecule details ›
Chain: A
Length: 523 amino acids
Theoretical weight: 57.54 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli M15
UniProt:
  • Canonical: G3I8R9 (Residues: 28-549; Coverage: 82%)
Gene names: GRP78, HSPA5, I79_019946
Sequence domains: Hsp70 protein

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 50.333Å b: 51.289Å c: 119.716Å
α: 90° β: 100.17° γ: 90°
R-values:
R R work R free
0.186 0.185 0.217
Expression system: Escherichia coli M15

Quick links

Citations

4 review citations

The Hsp70-Chaperone Machines in Bacteria.
Mayer MP. (2021)
3 more

3 mentions without citation

Calcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes.
Preissler et al. (2020)
2 more