› 7a7e

X-ray diffraction
2.8Å resolution

Structure of a delta-N mutant - E232start - of PA1120 (TpbB or YfiN) from Pseudomonas aeruginosa (PAO1) comprising only the GGDEF domain

Released:
DOI: 10.2210/pdb7a7e/pdb
Model geometry
Fit model/data
PDB entry 7a7e coloured by chain, front view.
PDB entry 7a7e coloured by chain, side view.
PDB entry 7a7e coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts.
Mantoni F, Scribani Rossi C, Paiardini A, Di Matteo A, Cappellacci L, Petrelli R, Ricciutelli M, Paone A, Cutruzzolà F, Giardina G, Rinaldo S
OpenAccess logo Life (Basel) 11 (2021)
PMID: 33418960

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diguanylate cyclase TpbB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 206 amino acids
Theoretical weight: 22.56 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I4L5 (Residues: 233-435; Coverage: 47%)
Gene names: PA1120, tpbB, yfiN
Sequence domains: Diguanylate cyclase, GGDEF domain

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 79.642Å b: 85.104Å c: 126.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Characterisation of sequence-structure-function space in sensor-effector integrators of phytochrome-regulated diguanylate cyclases.
Böhm et al. (2022)
3 more

1 mention without citation

Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts.
Mantoni et al. (2021)