› 7a8w

X-ray diffraction
2.15Å resolution

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikC with an engineered HMA domain of Pikp-1 from rice (Oryza sativa)

Released:
DOI: 10.2210/pdb7a8w/pdb
PDB entry 7a8w coloured by chain, front view.
PDB entry 7a8w coloured by chain, side view.
PDB entry 7a8w coloured by chain, top view.
Source organisms:
Primary publication:
The allelic rice immune receptor Pikh confers extended resistance to strains of the blast fungus through a single polymorphism in the effector binding interface.
De la Concepcion JC, Maidment JHR, Longya A, Xiao G, Franceschetti M, Banfield MJ
PLoS Pathog 17 e1009368 (2021)
PMID: 33647072

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112707 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HMA domain-containing protein Chains: AAA, BBB, DDD, EEE
Molecule details ›
Chains: AAA, BBB, DDD, EEE
Length: 81 amino acids
Theoretical weight: 8.62 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: D5L9G5 (Residues: 183-261; Coverage: 7%)
Gene names: Pi-km1, Pikh-1
AVR-Pik protein Chains: CCC, FFF
Molecule details ›
Chains: CCC, FFF
Length: 93 amino acids
Theoretical weight: 10.85 KDa
Source organism: Pyricularia oryzae 70-15
Expression system: Escherichia coli
UniProt:
  • Canonical: G4MXW3 (Residues: 22-113; Coverage: 100%)
Gene name: MGG_15972

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 66.784Å b: 80.205Å c: 105.683Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.272
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Resistance that stacks up: engineering rust and mildew disease control in the cereal crops wheat and barley.
Dracatos et al. (2023)
2 more

2 mentions without citation

The allelic rice immune receptor Pikh confers extended resistance to strains of the blast fungus through a single polymorphism in the effector binding interface.
De la Concepcion et al. (2021)
1 more