› 7ac0

X-ray diffraction
2.18Å resolution

Epoxide hydrolase CorEH without ligand

Released:
DOI: 10.2210/pdb7ac0/pdb
PDB entry 7ac0 coloured by chain, front view.
PDB entry 7ac0 coloured by chain, side view.
PDB entry 7ac0 coloured by chain, top view.
Source organism: Corynebacterium sp. C12
Primary publication:
Promiscuous Dehalogenase Activity of the Epoxide Hydrolase CorEH from Corynebacterium sp. C12
Schuiten ED, Badenhorst CPS, Palm GJ, Berndt L, Lammers M, Mican J, Bednar D, Damborsky J, Bornscheuer UT
ACS Catalysis 11 6113-6120 (2021)

Function and Biology Details

Reaction catalysed: 
An epoxide + H(2)O = a glycol
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129417 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble epoxide hydrolase Chains: AAA, BBB, CCC, DDD, EEE, FFF, GGG, HHH
Molecule details ›
Chains: AAA, BBB, CCC, DDD, EEE, FFF, GGG, HHH
Length: 304 amino acids
Theoretical weight: 34.17 KDa
Source organism: Corynebacterium sp. C12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O52866 (Residues: 1-286; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21212
Unit cell:
a: 140.016Å b: 156.57Å c: 112.581Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.226
Expression system: Escherichia coli BL21(DE3)

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