› 7acs

Solution NMR

The SARS-CoV-2 nucleocapsid phosphoprotein N-terminal domain in complex with 7mer dsRNA

Released:
DOI: 10.2210/pdb7acs/pdb
PDB entry 7acs coloured by chain, ensemble of 30 models, front view.
PDB entry 7acs coloured by chain, ensemble of 30 models, side view.
PDB entry 7acs coloured by chain, ensemble of 30 models, top view.
Source organism: Severe acute respiratory syndrome coronavirus 2
Primary publication:
Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein.
Dinesh DC, Chalupska D, Silhan J, Koutna E, Nencka R, Veverka V, Boura E
OpenAccess logo PLoS Pathog 16 e1009100 (2020)
PMID: 33264373

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145001 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15.13 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DTC9 (Residues: 44-180; Coverage: 33%)
Gene name: N
Sequence domains: Coronavirus nucleocapsid
RNA (5'-R(P*CP*AP*CP*UP*GP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.18 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Not provided
RNA (5'-R(P*GP*UP*CP*AP*GP*UP*G)-3') Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 79%
Refinement method: molecular dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

Structural insights into SARS-CoV-2 proteins.
Arya et al. (2021)
24 more

15 mentions without citation

Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein.
Dinesh et al. (2020)
14 more