› 7agv

X-ray diffraction
1.85Å resolution

High-resolution structure of the K+/H+ antiporter subunit KhtT in complex with c-di-AMP

Released:
DOI: 10.2210/pdb7agv/pdb
PDB entry 7agv coloured by chain, front view.
PDB entry 7agv coloured by chain, side view.
PDB entry 7agv coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
c-di-AMP, a likely master regulator of bacterial K+ homeostasis machinery, activates a K+ exporter.
Cereija TB, Guerra JPL, Jorge JMP, Morais-Cabral JH
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33790011

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
K(+)/H(+) antiporter subunit KhtT Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 168 amino acids
Theoretical weight: 18.94 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O07535 (Residues: 2-165; Coverage: 99%)
Gene names: BSU09860, khtT, yhaT
Sequence domains: TrkA-C domain

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ACT 12 x ACT
Ligand 2BA 4 x 2BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 72.945Å b: 127.598Å c: 81.881Å
α: 90° β: 95.8° γ: 90°
R-values:
R R work R free
0.206 0.204 0.242
Expression system: Escherichia coli

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Citations

4 review citations

Cyclic di-AMP, a multifaceted regulator of central metabolism and osmolyte homeostasis in Listeria monocytogenes.
Schwedt et al. (2023)
3 more

1 mention without citation

c-di-AMP, a likely master regulator of bacterial K+ homeostasis machinery, activates a K+ exporter.
Cereija et al. (2021)