› 7ahm

X-ray diffraction
3.14Å resolution

Low-resolution structure of the K+/H+ antiporter subunit KhtT in complex with c-di-AMP

Released:
DOI: 10.2210/pdb7ahm/pdb
PDB entry 7ahm coloured by chain, front view.
PDB entry 7ahm coloured by chain, side view.
PDB entry 7ahm coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
c-di-AMP, a likely master regulator of bacterial K+ homeostasis machinery, activates a K+ exporter.
Cereija TB, Guerra JPL, Jorge JMP, Morais-Cabral JH
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33790011

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
K(+)/H(+) antiporter subunit KhtT Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 168 amino acids
Theoretical weight: 18.94 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O07535 (Residues: 2-165; Coverage: 99%)
Gene names: BSU09860, khtT, yhaT
Sequence domains: TrkA-C domain

Ligands and Environments

1 bound ligand:
Ligand 2BA 2 x 2BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P4222
Unit cell:
a: 190.651Å b: 190.651Å c: 84.574Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.237 0.272
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The role of bacterial cyclic di-adenosine monophosphate in the host immune response.
Cheng et al. (2022)
3 more

1 mention without citation

c-di-AMP, a likely master regulator of bacterial K+ homeostasis machinery, activates a K+ exporter.
Cereija et al. (2021)