› 7alo

X-ray diffraction
1.8Å resolution

Structure of B*27:09/photoRL9

Released:
DOI: 10.2210/pdb7alo/pdb
PDB entry 7alo coloured by chain, front view.
PDB entry 7alo coloured by chain, side view.
PDB entry 7alo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Exchange catalysis by tapasin exploits conserved and allele-specific features of MHC-I molecules.
Lan H, Abualrous ET, Sticht J, Fernandez LMA, Werk T, Weise C, Ballaschk M, Schmieder P, Loll B, Freund C
Nat Commun 12 4236 (2021)
PMID: 34244493

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-104439 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 292 amino acids
Theoretical weight: 33.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2R7Z5J3 (Residues: 10-10, 25-301; Coverage: 78%)
Gene name: HLA-B
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Vasoactive intestinal polypeptide receptor 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P32241 (Residues: 400-408; Coverage: 2%)
Gene name: VIPR1

Ligands and Environments

5 bound ligands:
Ligand GOL 12 x GOL
Ligand TRS 2 x TRS
Ligand 1PE 1 x 1PE
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 45.2Å b: 69.61Å c: 82.54Å
α: 80.24° β: 88.362° γ: 89.877°
R-values:
R R work R free
0.226 0.223 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

The mode of action of tapasin on major histocompatibility class I (MHC-I) molecules.
Lan et al. (2023)
1 more

1 mention without citation

Exchange catalysis by tapasin exploits conserved and allele-specific features of MHC-I molecules.
Lan et al. (2021)