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› 7amb

X-ray diffraction
1.63Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 26.72 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P42212 (Residues: 2-232; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
1 modified residue:
Ligand PIA 4 x PIA

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: C2
Unit cell:
a: 142.3Å b: 134.5Å c: 51.62Å
α: 90° β: 106.11° γ: 90°
R-values:
R R work R free
0.151 0.151 0.19
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 citation in other articles

Rational Control of Off-State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement.
Adam et al. (2022)
1 more

PDB-REDO

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