› 7aml

Electron Microscopy
3.5Å resolution

RET/GDNF/GFRa1 extracellular complex Cryo-EM structure

Released:
DOI: 10.2210/pdb7aml/pdb
PDB entry 7aml coloured by chain, front view.
PDB entry 7aml coloured by chain, side view.
PDB entry 7aml coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
A two-site flexible clamp mechanism for RET-GDNF-GFRα1 assembly reveals both conformational adaptation and strict geometric spacing.
Adams SE, Purkiss AG, Knowles PP, Nans A, Briggs DC, Borg A, Earl CP, Goodman KM, Nawrotek A, Borg AJ, McIntosh PB, Houghton FM, Kjær S, McDonald NQ
Structure 29 694-708.e7 (2021)
PMID: 33484636
Related structures: EMD-11822

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-108625 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proto-oncogene tyrosine-protein kinase receptor Ret Chains: A, D
Molecule details ›
Chains: A, D
Length: 615 amino acids
Theoretical weight: 69.54 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A8E7C6 (Residues: 22-626; Coverage: 56%)
Gene names: c-ret, cret, etID315074.13, ret, ret1, wu:fd13h01
Sequence domains:
GDNF family receptor alpha Chains: B, E
Molecule details ›
Chains: B, E
Length: 353 amino acids
Theoretical weight: 39.74 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q98TT9 (Residues: 20-351; Coverage: 73%)
Gene names: gfra1a, gfralpha1a, zgc:110129
Sequence domains: GDNF/GAS1 domain
Glial cell line-derived neurotrophic factor Chains: C, F
Molecule details ›
Chains: C, F
Length: 101 amino acids
Theoretical weight: 11.57 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q98TU0 (Residues: 135-235; Coverage: 47%)
Gene name: gdnf
Sequence domains: Transforming growth factor beta like domain

Ligands and Environments

2 bound ligands:
Ligand CA 8 x CA
Ligand NAG 16 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-11822
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

RET receptor signaling: Function in development, metabolic disease, and cancer.
Takahashi M. (2022)
1 other article

4 mentions without citation

Cryo-electron Microscopic Analysis of Single-Pass Transmembrane Receptors.
Cai et al. (2022)
3 more