› 7aok

X-ray diffraction
1.87Å resolution

Crystal structure of CI2 mutant L49I

Released:
DOI: 10.2210/pdb7aok/pdb
Model geometry
Fit model/data
Data not analysed
PDB entry 7aok coloured by chain, front view.
PDB entry 7aok coloured by chain, side view.
PDB entry 7aok coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli.
Hamborg L, Granata D, Olsen JG, Roche JV, Pedersen LE, Nielsen AT, Lindorff-Larsen K, Teilum K
OpenAccess logo Commun Biol 4 980 (2021)
PMID: 34408246

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilisin-chymotrypsin inhibitor-2A Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.31 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01053 (Residues: 21-84; Coverage: 76%)
Sequence domains: Potato inhibitor I family

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P622
Unit cell:
a: 68.465Å b: 68.465Å c: 52.936Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.22 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

A dual-reporter system for investigating and optimizing protein translation and folding in E. coli.
Zutz et al. (2021)
4 more

1 mention without citation

Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli.
Hamborg et al. (2021)