› 7aom

X-ray diffraction
1.95Å resolution

Structure of NUDT15 in complex with Ganciclovir triphosphate

Released:
DOI: 10.2210/pdb7aom/pdb
Model geometry
Fit model/data
PDB entry 7aom coloured by chain, front view.
PDB entry 7aom coloured by chain, side view.
PDB entry 7aom coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NUDT15-mediated hydrolysis limits the efficacy of anti-HCMV drug ganciclovir.
Zhang SM, Rehling D, Jemth AS, Throup A, Landázuri N, Almlöf I, Göttmann M, Valerie NCK, Borhade SR, Wakchaure P, Page BDG, Desroses M, Homan EJ, Scobie M, Rudd SG, Berglund UW, Söderberg-Nauclér C, Stenmark P, Helleday T
Cell Chem Biol 28 1693-1702.e6 (2021)
PMID: 34192523

Function and Biology Details

Reaction catalysed: 
A nucleoside triphosphate + H(2)O = a nucleotide + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleotide triphosphate diphosphatase NUDT15 Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NV35 (Residues: 1-164; Coverage: 100%)
Gene names: MTH2, NUDT15
Sequence domains: NUDIX domain

Ligands and Environments

2 bound ligands:
Ligand RQ2 2 x RQ2
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 46.243Å b: 49.532Å c: 135.032Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.223
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Long-read HiFi sequencing of NUDT15: Phased full-gene haplotyping and pharmacogenomic allele discovery.
Scott et al. (2022)
3 more