› 7ap7

X-ray diffraction
1.15Å resolution

Structure of the W64R amyloidogenic variant of human lysozyme

Released:
DOI: 10.2210/pdb7ap7/pdb
Model geometry
Fit model/data
PDB entry 7ap7 coloured by chain, front view.
PDB entry 7ap7 coloured by chain, side view.
PDB entry 7ap7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterisation of the structural, dynamic and aggregation properties of the W64R amyloidogenic variant of human lysozyme.
Vettore N, Moray J, Brans A, Herman R, Charlier P, Kumita JR, Kerff F, Dobson CM, Dumoulin M
Biophys Chem 271 106563 (2021)
PMID: 33640796

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.69 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P61626 (Residues: 19-148; Coverage: 100%)
Gene names: LYZ, LZM
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 31.773Å b: 56.655Å c: 60.597Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.193
Expression system: Komagataella pastoris

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Citations

1 citations in other articles

Solvent-Induced Lag Phase during the Formation of Lysozyme Amyloid Fibrils Triggered by Sodium Dodecyl Sulfate: Biophysical Experimental and In Silico Study of Solvent Effects.
Zazeri et al. (2023)