› 7apn

X-ray diffraction
2Å resolution

Structure of Lipase TL from bulk agarose grown crystal

Released:
DOI: 10.2210/pdb7apn/pdb
PDB entry 7apn coloured by chain, front view.
PDB entry 7apn coloured by chain, side view.
PDB entry 7apn coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
Production of Cross-Linked Lipase Crystals at a Preparative Scale.
Fernández-Penas R, Verdugo-Escamilla C, Martínez-Rodríguez S, Gavira JA
Cryst Growth Des 21 1698-1707 (2021)
PMID: 34602865

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.34 KDa
Source organism: Thermomyces lanuginosus
Expression system: Aspergillus sp.
UniProt:
  • Canonical: O59952 (Residues: 23-291; Coverage: 98%)
Gene name: LIP
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand GOL 3 x GOL
Ligand PO4 2 x PO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P61
Unit cell:
a: 141.187Å b: 141.187Å c: 80.234Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.25
Expression system: Aspergillus sp.

Quick links

Citations

1 citations in other articles

Magnetite Mineralization inside Cross-Linked Protein Crystals.
Savchenko et al. (2023)

1 mention without citation

Production of Cross-Linked Lipase Crystals at a Preparative Scale.
Fernández-Penas et al. (2021)