› 7app

X-ray diffraction
1.7Å resolution

Structure of Lipase TL from capillary grown crystal in the presence of agarose

Released:
DOI: 10.2210/pdb7app/pdb
PDB entry 7app coloured by chain, front view.
PDB entry 7app coloured by chain, side view.
PDB entry 7app coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
Production of Cross-Linked Lipase Crystals at a Preparative Scale.
Fernández-Penas R, Verdugo-Escamilla C, Martínez-Rodríguez S, Gavira JA
Cryst Growth Des 21 1698-1707 (2021)
PMID: 34602865

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.34 KDa
Source organism: Thermomyces lanuginosus
Expression system: Aspergillus sp.
UniProt:
  • Canonical: O59952 (Residues: 23-291; Coverage: 98%)
Gene name: LIP
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand NA 3 x NA
Ligand FMT 15 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P61
Unit cell:
a: 137.851Å b: 137.851Å c: 79.942Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.128 0.127 0.154
Expression system: Aspergillus sp.

Quick links

Citations

1 citations in other articles

Magnetite Mineralization inside Cross-Linked Protein Crystals.
Savchenko et al. (2023)

1 mention without citation

Production of Cross-Linked Lipase Crystals at a Preparative Scale.
Fernández-Penas et al. (2021)