› 7aqa

X-ray diffraction
1.5Å resolution

Pseudomonas stutzeri nitrous oxide reductase mutant, H382A

Released:
DOI: 10.2210/pdb7aqa/pdb
PDB entry 7aqa coloured by chain, front view.
PDB entry 7aqa coloured by chain, side view.
PDB entry 7aqa coloured by chain, top view.
Source organism: Pseudomonas stutzeri
Primary publication:
A [3Cu:2S] cluster provides insight into the assembly and function of the CuZ site of nitrous oxide reductase.
Zhang L, Bill E, Kroneck PMH, Einsle O
Chem Sci 12 3239-3244 (2021)
PMID: 34164092

Function and Biology Details

Reaction catalysed: 
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrous-oxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 71.89 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19573 (Residues: 1-638; Coverage: 100%)
Gene name: nosZ
Sequence domains:

Ligands and Environments

10 bound ligands:
Ligand K7E 1 x K7E
Ligand B3P 2 x B3P
Ligand NA 1 x NA
Ligand CA 2 x CA
Ligand K 2 x K
Ligand CL 3 x CL
Ligand CUA 2 x CUA
Ligand PHE 1 x PHE
Ligand RUQ 1 x RUQ
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 68.824Å b: 76.834Å c: 108.513Å
α: 90° β: 93.47° γ: 90°
R-values:
R R work R free
0.158 0.156 0.194
Expression system: Escherichia coli BL21(DE3)

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