› 7aqd

Electron Microscopy
3.1Å resolution

Structure of the bacterial RQC complex (Translocating State)

Released:
DOI: 10.2210/pdb7aqd/pdb
PDB entry 7aqd coloured by chain, front view.
PDB entry 7aqd coloured by chain, side view.
PDB entry 7aqd coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Mimicry of Canonical Translation Elongation Underlies Alanine Tail Synthesis in RQC.
Filbeck S, Cerullo F, Paternoga H, Tsaprailis G, Joazeiro CAP, Pfeffer S
Mol Cell 81 104-114.e6 (2021)
PMID: 33259811
Related structures: EMD-11864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 32-mer (preferred)
PDBe Complex ID:
PDB-CPX-301308 (preferred)
Entry contents:
29 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (32 distinct):
Rqc2 homolog RqcH Chain: R
Molecule details ›
Chain: R
Length: 570 amino acids
Theoretical weight: 65.31 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: O34693 (Residues: 1-570; Coverage: 100%)
Gene names: BSU15640, rqcH, yloA
Sequence domains:
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 277 amino acids
Theoretical weight: 30.34 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
UniProt:
  • Canonical: P42919 (Residues: 1-277; Coverage: 100%)
Gene names: BSU01190, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Large ribosomal subunit protein uL4 Chain: E
Large ribosomal subunit protein uL5 Chain: F
Large ribosomal subunit protein uL6 Chain: G
Large ribosomal subunit protein uL11 Chain: I
Large ribosomal subunit protein uL13 Chain: J
Large ribosomal subunit protein uL14 Chain: K
Large ribosomal subunit protein uL15 Chain: L
Large ribosomal subunit protein uL16 Chain: M
Large ribosomal subunit protein bL17 Chain: N
Large ribosomal subunit protein uL18 Chain: O
Large ribosomal subunit protein bL20 Chain: Q
Large ribosomal subunit protein uL22 Chain: S
Large ribosomal subunit protein uL24 Chain: U
Large ribosomal subunit protein bL27 Chain: V
nascent polyalanine Chain: W
Large ribosomal subunit protein bL28 Chain: X
Large ribosomal subunit protein uL30 Chain: Z
Large ribosomal subunit protein bL19 Chain: a
Large ribosomal subunit protein bL32 Chain: b
Large ribosomal subunit protein bL33A Chain: c
Large ribosomal subunit protein bL34 Chain: d
Large ribosomal subunit protein bL35 Chain: e
Large ribosomal subunit protein bL36 Chain: f
Large ribosomal subunit protein bL21 Chain: g
Large ribosomal subunit protein uL23 Chain: h
Large ribosomal subunit protein uL29 Chain: i
23S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B
Ala-tRNA (A/P-site) Chain: T

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-11864

Quick links

Citations

6 review citations

Ribosome Rescue Pathways in Bacteria.
Müller et al. (2021)
5 more

2 mentions without citation

Ribosome-associated quality-control mechanisms from bacteria to humans.
Filbeck et al. (2022)
1 more