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› 7ark

Electron Microscopy
4.1Å resolution

LolCDE in complex with AMP-PNP in the closed NBD state

Released:
DOI: 10.2210/pdb7ark/pdb
PDB entry 7ark coloured by chain, front view.
PDB entry 7ark coloured by chain, side view.
PDB entry 7ark coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for bacterial lipoprotein relocation by the transporter LolCDE.
Tang X, Chang S, Zhang K, Luo Q, Zhang Z, Wang T, Qiao W, Wang C, Shen C, Zhang Z, Zhu X, Wei X, Dong C, Zhang X, Dong H
Nat Struct Mol Biol 28 347-355 (2021)
PMID: 33782615
Related structures: EMD-11885

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142348 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lipoprotein-releasing system transmembrane protein LolC Chain: C
Molecule details ›
Chain: C
Length: 399 amino acids
Theoretical weight: 43.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADC3 (Residues: 1-399; Coverage: 100%)
Gene names: JW5161, b1116, lolC, ycfU
Sequence domains:
Lipoprotein-releasing system transmembrane protein LolE Chain: E
Molecule details ›
Chain: E
Length: 414 amino acids
Theoretical weight: 45.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P75958 (Residues: 1-414; Coverage: 100%)
Gene names: JW1104, b1118, lolE, ycfW
Sequence domains:
Lipoprotein-releasing system ATP-binding protein LolD Chains: D, F

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.1Å
Relevant EMDB volumes: EMD-11885
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Bacterial Lipoprotein Posttranslational Modifications. New Insights and Opportunities for Antibiotic and Vaccine Development.
Smithers et al. (2021)
3 more

2 mentions without citation

Cryo-EM structures of LolCDE reveal the molecular mechanism of bacterial lipoprotein sorting in Escherichia coli.
Bei et al. (2022)
1 more