› 7arp

X-ray diffraction
1.78Å resolution

Native L-2-haloacid dehalogenase from Zobellia galactanivorans

Released:
DOI: 10.2210/pdb7arp/pdb
Model geometry
Fit model/data
PDB entry 7arp coloured by chain, front view.
PDB entry 7arp coloured by chain, side view.
PDB entry 7arp coloured by chain, top view.
Source organism: Zobellia galactanivorans
Primary publication:
X-ray structure and mechanism of ZgHAD, a L-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans.
Grigorian E, Roret T, Czjzek M, Leblanc C, Delage L
OpenAccess logo Protein Sci e4540 (2022)
PMID: 36502283

Function and Biology Details

Reaction catalysed: 
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-haloacid dehalogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 26.38 KDa
Source organism: Zobellia galactanivorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0L7V6 (Residues: 2-227; Coverage: 100%)
Gene names: dhlB, zobellia_4183
Sequence domains: haloacid dehalogenase-like hydrolase

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand SCN 2 x SCN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 59.535Å b: 68.826Å c: 103.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

X-ray structure and mechanism of ZgHAD, a l-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans.
Grigorian et al. (2023)