› 7aue

Electron Microscopy
2.97Å resolution

Melanocortin receptor 4 (MC4R) Gs protein complex

Released:
DOI: 10.2210/pdb7aue/pdb
PDB entry 7aue coloured by chain, front view.
PDB entry 7aue coloured by chain, side view.
PDB entry 7aue coloured by chain, top view.
Source organisms:
Primary publication:
Structure reveals the activation mechanism of the MC4 receptor to initiate satiation signaling.
Israeli H, Degtjarik O, Fierro F, Chunilal V, Gill AK, Roth NJ, Botta J, Prabahar V, Peleg Y, Chan LF, Ben-Zvi D, McCormick PJ, Niv MY, Shalev-Benami M
Science 372 808-814 (2021)
PMID: 33858992
Related structures: EMD-11927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225596 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Melanocortin receptor 4 Chain: R
Molecule details ›
Chain: R
Length: 350 amino acids
Theoretical weight: 38.97 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32245 (Residues: 1-332; Coverage: 100%)
Gene name: MC4R
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 43.19 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 39-394; Coverage: 87%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 357 amino acids
Theoretical weight: 39.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 256 amino acids
Theoretical weight: 27.34 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
AMINOSERINE Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.11 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
3 modified residues:
Ligand DAL 1 x DAL
Ligand DPN 1 x DPN
Ligand CY3 1 x CY3

Experiments and Validation Details

Entry percentile scores
Resolution: 2.97Å
Relevant EMDB volumes: EMD-11927
Expression systems:
  • Spodoptera frugiperda
  • Trichoplusia ni
  • Not provided

Quick links

Citations

7 review citations

Obesity, POMC, and POMC-processing Enzymes: Surprising Results From Animal Models.
Lindberg et al. (2021)
6 more

8 mentions without citation

Immunomodulatory Role of Neuropeptides in the Cornea.
Puri et al. (2022)
7 more