› 7av7

X-ray diffraction
2.9Å resolution

Crystal structure of S-nitrosylated nitrosoglutathione reductase(GSNOR)from Chlamydomonas reinhardtii, in complex with NAD+

Released:
DOI: 10.2210/pdb7av7/pdb
PDB entry 7av7 coloured by chain, front view.
PDB entry 7av7 coloured by chain, side view.
PDB entry 7av7 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structural and functional insights into nitrosoglutathione reductase from Chlamydomonas reinhardtii.
Tagliani A, Rossi J, Marchand CH, De Mia M, Tedesco D, Gurrieri L, Meloni M, Falini G, Trost P, Lemaire SD, Fermani S, Zaffagnini M
Redox Biol 38 101806 (2021)
PMID: 33316743

Function and Biology Details

Reaction catalysed: 
S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-(hydroxymethyl)glutathione dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 378 amino acids
Theoretical weight: 40.48 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2K3D6R4 (Residues: 1-378; Coverage: 100%)
Gene name: CHLRE_12g543400v5
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 6 x NAD
2 bound ligands:
Ligand ZN 12 x ZN
Ligand CL 1 x CL
1 modified residue:
Ligand SNC 6 x SNC

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 78.386Å b: 143.294Å c: 206.271Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.18 0.257
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Quantitative Proteome Profiling of a S-Nitrosoglutathione Reductase (GSNOR) Null Mutant Reveals a New Class of Enzymes Involved in Nitric Oxide Homeostasis in Plants.
Treffon et al. (2021)
2 more

2 mentions without citation

Structural and functional insights into nitrosoglutathione reductase from Chlamydomonas reinhardtii.
Tagliani et al. (2021)
1 more