› 7b0y

Electron Microscopy
3.6Å resolution

Structure of a transcribing RNA polymerase II-U1 snRNP complex

Released:
DOI: 10.2210/pdb7b0y/pdb
PDB entry 7b0y coloured by chain, front view.
PDB entry 7b0y coloured by chain, side view.
PDB entry 7b0y coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a transcribing RNA polymerase II-U1 snRNP complex.
Zhang S, Aibara S, Vos SM, Agafonov DE, Lührmann R, Cramer P
Science 371 305-309 (2021)
PMID: 33446560
Related structures: EMD-11972

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 25-mer (preferred)
PDBe Complex ID:
PDB-CPX-300251 (preferred)
Entry contents:
21 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct RNA molecules
Macromolecules (25 distinct):
DNA-directed RNA polymerase II subunit RPB1 Chain: A
Molecule details ›
Chain: A
Length: 1970 amino acids
Theoretical weight: 217.45 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: P11414 (Residues: 1-1970; Coverage: 100%)
Gene name: POLR2A
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 1174 amino acids
Theoretical weight: 134.04 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: A0A0B8RVL1 (Residues: 1-1174; Coverage: 100%)
Gene name: POLR2B
Sequence domains:
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerase II subunit RPB4 Chain: D
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase II subunit RPB7 Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
U1 small nuclear ribonucleoprotein 70 kDa Chain: b
U1 small nuclear ribonucleoprotein A Chain: c
Small nuclear ribonucleoprotein Sm D2 Chain: e
Small nuclear ribonucleoprotein F Chain: f
Small nuclear ribonucleoprotein E Chain: g
Small nuclear ribonucleoprotein G Chain: h
Small nuclear ribonucleoprotein Sm D3 Chain: i
Small nuclear ribonucleoprotein-associated protein Chain: j
Small nuclear ribonucleoprotein Sm D1 Chain: k
Non-template DNA Chain: N
Template DNA Chain: T
145-nt RNA Chain: P
U1 snRNA Chain: a

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.6Å
Relevant EMDB volumes: EMD-11972

Quick links

Citations

17 review citations

Nuclear compartmentalization as a mechanism of quantitative control of gene expression.
Bhat et al. (2021)
16 more

2 mentions without citation

Structural advances in transcription elongation.
Mohamed et al. (2022)
1 more