› 7b1i

X-ray diffraction
1.9Å resolution

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikF with the HMA domain of OsHIPP19 from rice (Oryza sativa)

Released:
DOI: 10.2210/pdb7b1i/pdb
PDB entry 7b1i coloured by chain, front view.
PDB entry 7b1i coloured by chain, side view.
PDB entry 7b1i coloured by chain, top view.
Source organisms:
Primary publication:
Multiple variants of the fungal effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19, providing a foundation to engineer plant defense.
Maidment JHR, Franceschetti M, Maqbool A, Saitoh H, Jantasuriyarat C, Kamoun S, Terauchi R, Banfield MJ
J Biol Chem 296 100371
PMID: 33548226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103938 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HMA domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 78 amino acids
Theoretical weight: 8.34 KDa
Source organism: Oryza sativa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01IL6 (Residues: 2-77; Coverage: 62%)
Gene names: OSIGBa0128P10.9, OSIGBa0150F01.4
Avr-Pik Chain: C
Molecule details ›
Chain: C
Length: 94 amino acids
Theoretical weight: 10.86 KDa
Source organism: Pyricularia oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A219T3Y8 (Residues: 22-113; Coverage: 100%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 29.783Å b: 53.782Å c: 98.026Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.233
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Advancement in the Breeding, Biotechnological and Genomic Tools towards Development of Durable Genetic Resistance against the Rice Blast Disease.
Sahu et al. (2022)
5 more

5 mentions without citation

Exploiting Structural Modelling Tools to Explore Host-Translocated Effector Proteins.
Amoozadeh et al. (2021)
4 more