› 7b1x

X-ray diffraction
2.3Å resolution

Crystal structure of cold-active esterase PMGL3 from permafrost metagenomic library

Released:
DOI: 10.2210/pdb7b1x/pdb
PDB entry 7b1x coloured by chain, front view.
PDB entry 7b1x coloured by chain, side view.
PDB entry 7b1x coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Structural and Biochemical Characterization of a Cold-Active PMGL3 Esterase with Unusual Oligomeric Structure.
Boyko KM, Kryukova MV, Petrovskaya LE, Kryukova EA, Nikolaeva AY, Korzhenevsky DA, Lomakina GY, Novototskaya-Vlasova KA, Rivkina EM, Dolgikh DA, Kirpichnikov MP, Popov VO
Biomolecules 11 (2021)
PMID: 33466452

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
esterase PMGL3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 33.44 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: C2
Unit cell:
a: 103.8Å b: 98.4Å c: 75.2Å
α: 90° β: 128.1° γ: 90°
R-values:
R R work R free
0.165 0.162 0.231
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Identification and Biochemical Characterization of a Novel Hormone-Sensitive Lipase Family Esterase Est19 from the Antarctic Bacterium Pseudomonas sp. E2-15.
Liu et al. (2021)
3 more

3 mentions without citation

Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues.
Matinja et al. (2022)
2 more