› 7b2q

Electron Microscopy
3.76Å resolution

Cryo-EM structure of complement C4b in complex with nanobody B12

Released:
DOI: 10.2210/pdb7b2q/pdb
PDB entry 7b2q coloured by chain, front view.
PDB entry 7b2q coloured by chain, side view.
PDB entry 7b2q coloured by chain, top view.
Source organisms:
Primary publication:
Multifaceted Activities of Seven Nanobodies against Complement C4b.
De la O Becerra KI, Oosterheert W, van den Bos RM, Xenaki KT, Lorent JH, Ruyken M, Schouten A, Rooijakkers SHM, van Bergen En Henegouwen PMP, Gros P
J Immunol 208 2207-2219 (2022)
PMID: 35428691
Related structures: EMD-11990

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209600 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Complement C4 beta chain Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 71.76 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 20-675; Coverage: 38%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Complement C4-A alpha chain Chain: B
Molecule details ›
Chain: B
Length: 767 amino acids
Theoretical weight: 84.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 680-1446; Coverage: 45%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Complement C4 gamma chain Chain: C
Molecule details ›
Chain: C
Length: 291 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 1454-1744; Coverage: 17%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Anti-C4b nanobody B12 Chain: D
Molecule details ›
Chain: D
Length: 139 amino acids
Theoretical weight: 15.27 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.76Å
Relevant EMDB volumes: EMD-11990
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Complement System in Kidney Transplantation.
Santarsiero et al. (2023)
1 other article

1 mention without citation

Multifaceted Activities of Seven Nanobodies against Complement C4b.
De la O Becerra et al. (2022)