› 7b3s

X-ray diffraction
1.85Å resolution

OXA-10 beta-lactamase with S67Dha modification

Released:
DOI: 10.2210/pdb7b3s/pdb
PDB entry 7b3s coloured by chain, front view.
PDB entry 7b3s coloured by chain, side view.
PDB entry 7b3s coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Studies on enmetazobactam clarify mechanisms of widely used β-lactamase inhibitors.
Lang PA, Raj R, Tumber A, Lohans CT, Rabe P, Robinson CV, Brem J, Schofield CJ
Proc Natl Acad Sci U S A 119 e2117310119 (2022)
PMID: 35486701

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.68 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P14489 (Residues: 20-265; Coverage: 100%)
Gene names: bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain

Ligands and Environments

3 bound ligands:
Ligand CO2 2 x CO2
Ligand SO4 4 x SO4
Ligand NA 3 x NA
2 modified residues:
Ligand DHA 2 x DHA
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 48.896Å b: 97.205Å c: 125.898Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.203
Expression system: Escherichia coli

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Citations

3 review citations

The Role of the Respiratory Microbiome in the Pathogenesis of Aspiration Pneumonia: Implications for Diagnosis and Potential Therapeutic Choices.
Vallianou et al. (2023)
2 more

1 mention without citation

Studies on enmetazobactam clarify mechanisms of widely used β-lactamase inhibitors.
Lang et al. (2022)