› 7b4b

X-ray diffraction
1.76Å resolution

Structural basis of reactivation of oncogenic p53 mutants by a small molecule: methylene quinuclidinone (MQ). Human p53DBD-R273C mutant bound to MQ: R273C-MQ (I)

Released:
DOI: 10.2210/pdb7b4b/pdb
PDB entry 7b4b coloured by chain, front view.
PDB entry 7b4b coloured by chain, side view.
PDB entry 7b4b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of reactivation of oncogenic p53 mutants by a small molecule: methylene quinuclidinone (MQ).
Degtjarik O, Golovenko D, Diskin-Posner Y, Abrahmsén L, Rozenberg H, Shakked Z
Nat Commun 12 7057 (2021)
PMID: 34862374

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular tumor antigen p53 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 200 amino acids
Theoretical weight: 22.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand QN8 4 x QN8
Ligand QNN 6 x QNN
Ligand EDO 4 x EDO
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 68.73Å b: 70.686Å c: 85.107Å
α: 90° β: 90.29° γ: 90°
R-values:
R R work R free
0.18 0.18 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Drugging p53 in cancer: one protein, many targets.
Hassin et al. (2023)
11 more