› 7b6b

X-ray diffraction
1.41Å resolution

The carbohydrate binding module family 48 (CBM48) and carboxy-terminal carbohydrate esterase family 1 (CE1) domains of the multidomain esterase DmCE1B from Dysgonomonas mossii in complex with methyl ferulate

Released:
DOI: 10.2210/pdb7b6b/pdb
PDB entry 7b6b coloured by chain, front view.
PDB entry 7b6b coloured by chain, side view.
PDB entry 7b6b coloured by chain, top view.
Source organism: Dysgonomonas mossii DSM 22836
Primary publication:
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases.
Kmezik C, Mazurkewich S, Meents T, McKee LS, Idström A, Armeni M, Savolainen O, Brändén G, Larsbrink J
J Biol Chem 296 100500
PMID: 33667545

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 43.36 KDa
Source organism: Dysgonomonas mossii DSM 22836
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F8X1N1 (Residues: 292-656; Coverage: 57%)
Gene name: HMPREF9456_02279
Sequence domains: Putative esterase

Ligands and Environments

3 bound ligands:
Ligand SZQ 2 x SZQ
Ligand CL 1 x CL
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P21
Unit cell:
a: 73.258Å b: 50.511Å c: 90.148Å
α: 90° β: 100.137° γ: 90°
R-values:
R R work R free
0.162 0.161 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Diet-gut microbiome interaction and ferulic acid bioavailability: implications on neurodegenerative disorders.
Kunnummal et al. (2023)
7 other articles

1 mention without citation

A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases.
Kmezik et al. (2021)