› 7b7c

X-ray diffraction
1.55Å resolution

Room temperature X-ray structure of perdeuterated PLL lectin in complex with L-fucose

Released:
DOI: 10.2210/pdb7b7c/pdb
PDB entry 7b7c coloured by chain, front view.
PDB entry 7b7c coloured by chain, side view.
PDB entry 7b7c coloured by chain, top view.
Source organism: Photorhabdus laumondii
Primary publication:
Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions.
Gajdos L, Blakeley MP, Kumar A, Wimmerová M, Haertlein M, Forsyth VT, Imberty A, Devos JM
Structure 29 1003-1013.e4 (2021)
PMID: 33765407

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bulb-type lectin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 41.94 KDa
Source organism: Photorhabdus laumondii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7N8J0 (Residues: 8-375; Coverage: 98%)
Gene name: plu0732
Sequence domains: Repeat of unknown function (DUF346)

Ligands and Environments

2 bound ligands:
Ligand FUL 4 x FUL
Ligand FUC 2 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I222
Unit cell:
a: 72.62Å b: 89.07Å c: 159.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.145 0.162
Expression system: Escherichia coli

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Citations

4 citation in other articles

Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding.
Gajdos et al. (2022)
3 more